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The three individual Projects and four Cores revolve around the development,
application, interpretation, and presentation of high resolution multidimensional
images of the developing nervous system obtained on several model systems
both in vitro and in vivo. The collaborators bring a number of different
philosophies and types of expertise: computer graphics, modeling and control
theory; mapping brain physiology/function and cortical learning; embryology,
cell and molecular biology, and digital optical microscopy; microscopic
resolution MRI.
Multi-Modal Imaging and Analysis of Neuronal Connectivity Project [Connectivity
Project]
Scott Fraser and Jerry Solomon
The goal of this Project is to develop
new strategies for collecting data and new computer software capable of
melding data of different resolutions, depths, and modality. These developments
will be driven by experiments designed to follow the patterning of axons
and cell bodies in the developing central nervous system (CNS). Imaging
CNS development in intact animals is fraught with difficulties. It requires:
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high resolution to follow the fine axonal processes of newly born neurons,
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the ability to image deep within tissue where key events take place, and
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a wide field of view to put the events into their proper context.
No single imaging modality offers the combination of attributes required
to meet these challenges. Therefore, this Project melds data from different
imaging modalities to synthesize the required views into neuronal development.
Cells will be labeled with axon tracing agents that are visible by both
light and MRI microscopy. Laser scanning confocal microscopy, two-photon
microscopy, and MRI microscopy performed on the same labeled cells in the
same animals will generate images at progressively greater depths in the
specimen, but different resolutions. Jerry Solomon, a new Co-PI on this
Project, will spearhead the computational effort to develop and implement
hierarchical multi-resolution image matching software to permit the merging
of high resolution light microscopy images with low to moderate resolution
MRI data. These software tools will be generally useful in all endeavors
requiring different imaging modalities, temporal resolution, spatial resolution,
and depth of penetration - not unusual circumstances in the neurosciences.
The resulting information will offer uniquely integrated views into both
the short-range and long-range aspects of neuronal patterning in the developing
CNS.
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